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| Nicotinic acid for Far UV Absorbance/Transmission |
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Nicotinic Acid – UV Absorbance and Linearity
Description: Nicotinic acid in 0.1M hydrochloric acid.
Primary Usage: Testing absorbance scale and linearity in the UV region.
Useable range: 210nm to 270nm, instruments with a SBW of 2 nm or less.
Physical Configuration: UV quartz cells that have been permanently heat sealed.
RM-UVA (1A2A3A4A) set: (see MSDS)
Consists of: one blank (0.1M hydrochloric acid) and four increasing concentrations, with nominal values of 6mg/l, 12mg/l, 18mg/l and 24mg/l. |
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The use of nicotinic acid solvated in dilute hydrochloric acid is a well documented method for the validation of the absorbance scale and linearity of a spectrophotometer in the Far UV region.
When prepared in 0.1M hydrochloric acid, nicotinic acid gives a spectral scan containing characteristic peaks at approx. 210nm and 260nm. Within the concentration range 5-25 mg/l, if the absorbance scale of a narrow SBW (Ž 2 nm) spectrophotometer is linear, the apparent absorbances of a series of concentrations will be a linear function of concentration, at a specified SBW. |
Typical values obtained:
| Wavelength |
213nm |
261nm |
| 6mg/l |
0.240 |
0.281 |
| 12mg/l |
0.477 |
0.556 |
| 18mg/l |
0.730 |
0.849 |
| 24mg/l |
0.988 |
1.149 |
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For the measurement of this fundamental parameter, Starna® offers you the choice of sealed cell materials. Please note that your selection process should not only include the wavelength(s) required, but also the spectral bandwidth (SBW) of your spectrophotometer. Use of the reference materials outside the SBW range detailed is not excluded, but variations from the certified values may be observed |
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Calibration procedure:
Traceability:
• Primary instrumental linearity is established using the Double Aperture method.
• Additional traceability links to NIST primary materials are established using SRM 935a potassium dichromate, SRM 930e and SRM 1930 neutral density glass filters. |
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Use:
• All appropriate fundamental parameters and
procedures relating to measurement, handling
and storage are fully documented on the certificate
supplied with each Certified Reference Material |
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