FAQ's Cuvette Matching

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Cell Matching

What does matching mean?

Matching of cells refers to the degree to which absorption cells give similar absorbance or transmission reading when empty or filled with water. The practice was started early in the history of spectrophotometer cell manufacturing and absorption instruments were single beam (lacking the ability to automatically adjust for a blank). As high quality cells from major manufacturers like Starna have become the norm and instruments have improved the concept of matching has become less important. A poor cell can appear matched because measuring a cell with no sample does not test the accuracy of the pathlength nor the dimensional quality of the windows. The important elements of cell quality are listed below with the specifications that you can expect from Starna cells.

PhysicalandChemical Characteristics

Quality Parameter	Specification
Parallelity of Windows	Better than 3 minutes of arc
The windows must be parallel so that the pathlength remains static over the entire cell Window.
Flatness of Windows	less than 4 Newton Fringes
The windows must be as flat as possible so that the light is not focused, reflected or refracted.
Window Polish	60/40 scratch/dig
The windows must be polished to a high tolerance to keep light dispersion to a minimum Quality Parameter

High tolerance pathlength
The distance between the interior of the cell's windows (the pathlength) must be maintained to a high tolerance. The table below specifies the maximum tolerance for a Starna cell. Due to the characteristics of each material, the tolerances are different for each material. Window Material

Window Material	Pathlength Range	Pathlength Tolerance
Glass	up to 20 mm	+/- 0.1 mm
Glass	30 to 100 mm	+/- 0.2 mm
Special Optical Glass	up to 20 mm	+/- 0.01 mm
Special Optical Glass	30 to 100 mm	+/- 0.02 mm
Quartz	up to 0.05 mm	+/- 0.001 mm
Quartz	0.1 to 0.4 mm	+/- 0.005 mm
Quartz	0.5 to 100 mm	+/- 0.01 mm

All of the above factors are critical to the performance of a spectrophotometer and a fluorimeter cell. When all parameters are maintained to a high degree the cell is "matched" by the fact that there is little difference in any of the cells of the same physical configuration, material and pathlength. We manufacture cells to such a high tolerance that we provide "better than matched".
Can Fluorimeter Cells be matched?
By definition matching is the testing of absorbtion directly through the pathlength of a cell and does not address any parameters used in fluorimetry. Using a high quality cell that is constructed of "background fluorescence free" quartz is much more important. Our Spectrosil quartz is an excellent material for fluorescence.

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