Measurements by fluorescence spectroscopy are subject to many more instrumental and physical variables than absorption measurements. Due to these effects, the same sample may not give identical results with different instruments even if they are of similar design. Physical effects such as quenching or self-absorption may lead to departures from expected concentration/emission relationships over quite narrow concentration ranges. For these reasons it may be impossible to produce universal “absolute” reference materials, as certified values may be instrument dependent. Provided homogeneity and stability can be established, however, reference materials can be produced that allow users to monitor the performance of a spectrofluorometer over time and can also indicate the relative performance of different instruments. Such references are referred to as “Reference Materials” (RMs) or Quality Control Materials (QCMs). Starna produce a range of such materials under the same tight quality control procedures as are applied to their CRMs.
For the reasons outlined above, instrument qualification may only be valid for a particular instrument and within a relatively narrow range of operating parameters – indeed it can even be method specific. The references listed below will cater for many routine situations, but Starna can also design and supply fluorescence Reference Materials tailored to individual needs.
used to calibrate the spectral response of a spectrofluorometer and as a linearity check.
used to determine the signal-to-noise ratio of a fluorescence spectrophotometer and hence its ultimate sensitivity.
spectral response references, traceable to NIST Fluorescence SRMs NEW!
used to confirm measurements of transient fluorescentsignals.
used in fluorescence instrumentation for spectral correction
used to determine the fluorescence quantum yield of a sample
for spectrofluorometer wavelength qualification andas a routine check of spectrofluorometer performance.
for spectrofluorometer wavelength qualification
qualification of fluorescence-based plate readers for fluorescence intensity and well-to-well reproducibility NEW!