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  • Wavelength
  • Holmium Oxide Liquid (240-650 nm)

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52/54 Fowler Road
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Last updated 6 June 2018

 

Technique


  • UV-Visible Spectroscopy
  • Visible Spectroscopy
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Visible References


  • Absorbance
    • Neutral Density Filters
    • Metal-on-Quartz Neutral Density Filters (250-635 nm)
    • Starna Green (Broadband SBW instruments) (250-650 nm)
    • Combined Holmium/Neutral Density glass (360 - 640 nm)
    • Combined Didymium/Neutral Density glass (430 - 890 nm)
  • Wavelength
    • Holmium Oxide Liquid (240-650 nm)
    • Didymium Oxide Liquid (290-870 nm)
    • Holmium Oxide Glass (270-640 nm)
    • Didymium Glass (430 nm to 890 nm)
    • Combined Holmium/Neutral Density glass (360 - 640 nm)
    • Combined Didymium/Neutral Density glass (430 - 890 nm)
    • Starna Green (Broadband SBW instruments) (250-650 nm)
    • Samarium Oxide Liquid (230-560 nm)

Holmium Oxide

Purpose

Purpose

This Reference Material can be used to qualify the wavelength calibration, in the ultraviolet and visible regions of the spectrum (240 nm - 650 nm) of spectrophotometers with spectral bandwidths of 3 nm or less. It is accepted for this purpose by most Pharmacopoeias and Standardisation Bodies.

 

 

Description and Discussion

Description and Discussion

Holmium oxide (4% m/v) in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette.

The use of holmium oxide solvated in perchloric acid is an established and well recognised method for the validation of the wavelength scale of a spectrophotometer in the UV and visible regions. Aqueous perchloric acid solvent is used as the solvent as the resulting Ho3+aq species is relatively stable to changes in temperature and concentration.

When prepared in perchloric acid, holmium oxide gives a spectral scan containing a series of 14 characteristic andwell-defined peaks covering the wavelength range from 240 to 650 nm.

Typical spectra

Typical spectra

Hollium-oxide

Approximate peak wavelength values (in nm) are:
241, 250, 278, 288, 334, 346, 361, 385, 417, 451, 468, 485, 537, 641

Note: The above values are for guidance only. Because the absorption bands are asymmetric, measured values will bespectral bandwidth dependent. The Calibration Certificate accompanying each Starna Holmium reference gives actual values measured at bandwidths of 0.10, 0.25, 0.50, 1.00, 1.50, 2.00, 3.00, 4.00 and 5.00 nm, and onlythese certified values should be used for instrument qualification. On request, Starna can provide certifiedvalues at other wavelengths and bandwidth values.

 
Catalogue Number

Catalogue Number

  Catalogue  Number

Holmium oxide liquid cell

RM-HL

Product enquiry

Product enquiry

Data sheet

Data sheet

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