User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry

 These cell-based reference materials are designed for the qualification of HPLC UV detectors that can accept conventional spectrophotometer cells, e.g. Waters.

Erbium Oxide Solution – Wavelength Qualification

UV-Vis - Micro-Plate Reader Liquid Filter Reference Plate 

Erbium oxide in perchloric acid gives a spectral scan containing 9 well defined peaks from 230 nm to 660 nm. In typical HPLC DAD qualification protocols (e.g Waters) the three peaks at 255, 379 and 522 nm are used for wavelength qualification.

 

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Propyl Parabenz Solution – Photometric Linearity Qualification 

 HPLC Linearity Reference – Propyl Parbenz Plate

Heat sealed cells containing Propyl Parabenz solution in methanol. Five concentrations between 5 mg/l and 30 mg/l. When measured against the supplied methanol blank at 257 nm, the graph of absorbance against concentration is linear.

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