UV-Visible spectroscopy is probably the most widely used instrumental techniques in analytical chemistry, especially in the life sciences. As such, it is one of the most regulated, by pharmacopoeias and quality assurance bodies world-wide. Starna supplies the world’s widest range of Certified Reference Materials for the qualification of UV-Visible Spectrophotometers to help instrument users achieve compliance – with confidence.

Ideally, references should be chosen whose calibrated values “bracket” the conditions to be used for analysis. 

 

Instrument Qualification Kits - Tools for Easier Compliance

Starna offer several Instrument Qualification Kits to assist with the qualification of a UV-Visible Spectrophotometer in the ultraviolet region of the spectrum. Kits are tailored to the requirements of Pharmacopoeias and other Quality Assurance bodies.


Absorbance References


Potassium Dichromate (235-430 nm) - the most widely used reference for absorbance accuracy 

Nicotinic Acid (210-260 nm) – for absorbance accuracy in the far UV

Starna Green (250-650 nm) - combined absorbance/wavelength accuracy in the UV and visible

Deep UV Reference (190-220 nm) – qualification in the deep UV: unique to Starna

Metal-on-Quartz Neutral Density Filters (250-635 nm) - durable filters with UV capability

Neutral Density Filters (440-635nm) - glass filters with absorbances from 0.04 A to 3.5 A

Combined Holmium/Neutral Density glass (360 - 640 nm)– 1 A absorbance check with wavelength calibration

Combined Didymium/Neutral Density glass (430 - 890 nm)– 1 A absorbance check with wavelength calibration


Wavelength References


Holmium Oxide Liquid (240-650 nm) - the most widely used reference for wavelength accuracy – accepted by all major Pharmacopoeias

Cerium Oxide Rare Earth liquid(200-300 nm) - a convenient way of qualifying spectrophotometers in the far UV.

Didymium Oxide Liquid (290-870 nm) – useful peaks in the NIR

Combined Holmium/Didymium Oxide Liquid (240 to 795 nm) – evenly spaced peaks over the range 

Starna Green (250-650 nm) - wavelength and absorbance for instruments with bandwidths >5nm

Holmium Oxide Glass (270-640 nm) – more robust and useful for routine checks  

Didymium Glass (430 - 890 nm) – more robust and useful for routine checks

Starna wide-range wavelength reference (335 - 1945 nm) - 20 peaks for wavelength calibration from UV to NIR. NEW!

Combined Holmium/Neutral Density glass (360 - 640 nm) – wavelength calibration with 1 A absorbance check

Combined Didymium/Neutral Density glass – (430 - 890 nm) - wavelength calibration with 1 A absorbance check

Deep UV Reference (190-220 nm) – qualification in the deep UV: unique to Starna

SamariumOxide Liquid (230 – 560 nm) – for the commonly used region from 200 nm to 500 nm 


Stray Light (Stray Radiant Energy) References


Stray Light Cut-off Filters –Specified Wavelength Method – (175-385 nm) accepted by most  Pharmacopoeias including USP

Stray Light Cut-off Filters – Filter Ratio Method(175-385 nm)  – recommended by US Pharmacopeia for higher absorbance values

Stray Light Glasses (220-440 nm) - convenient routine check for stray light


Resolution (Spectral Bandwidth) References


Toluene in Hexane  (265 – 270 nm) - the most widely used resolution reference – accepted by all major Pharmacopoeias

Toluene in Methanol (265 – 270 nm) - resolution check for Derivative Spectroscopy.

Benzene vapour (230-270 nm)  - resolution reference for instruments with spectral bandwidths less than 1 nm