User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry


This reference is useful as a resolution check for instruments with spectral bandwidths less than 1 nm.

Description and Discussion

0.01 ml benzene liquid sealed by heat fusion into a UV grade quartz cell. At room temperatures, the benzene is in the vapour phase.

The benzene vapour spectrum contains characteristic features that may or may not be resolved, dependent upon the spectrophotometer SBW. This is illustrated in the following spectra:

The peaks at 253.49 nm and 259.56 nm (arrowed) should be visible at a SBW of 0.2 nm or less.

Note: Benzene vapour will not work well with a photodiode array spectrophotometer as this instrument type does not measurea continuum and the peaks will usually not be sufficiently well resolved to be useable.


Typical spectra


Catalogue Number

  Catalogue Number 

Benzene vapour cell