User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry

 
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PRODUCT ENQUIRY
 
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FURTHER PRODUCT INFORMATION 
& HOW TO ORDER

 

This Reference Material can be used to qualify the absorbance and wavelength scales of spectrophotometers in the ultraviolet and visible regions of the spectrum (250 nm – 650 nm). It was specially developed for use with  instruments having wider bandwidths (up to 20 nm), but is a useful general purpose reference for all instruments.

The use of aqueous dye solutions is an established and well-recognised method for the validation of the absorbance scale and linearity of a spectrophotometer. Starna Green is a specially formulated dye solution with three broad but well-defined peaks that can be certified for both wavelength and absorbance value in the UV and visible regions, at bandwidths up to 20nm.

The reference is available at four increasing concentrations, with nominal concentration values of 25, 50, 75 and 100.

 

Starna-Green-Broadband-References-

starna-green 

Typical values obtained at the certified wavelengths are:

Nominal
concentration

Wavelength

258 nm

416 nm

630 nm

25

0.26

0.25

0.25

50

0.51

0.51

0.51

75

0.76

0.75

0.76

100

1.01

1.011

1.02

 

This product is also available in vials for qualifiying micro volume instruments

 

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO/IEC 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.