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These reference materials have been specially developed for the validation of DNA purity measurements when performed with the dedicated low volume (< 5 ul) and short path length (< 1 mm) instrumentation offered by several vendors for use in genomics and proteomics, when sample is often at a premium. They are also ideal for qualifying conventional instruments when they are used with ultra- low volume cells such as the Starna DMV-Bio cell.

The references are supplied in vials with screw-cap closure for ease of handling, and from which it is easy to transfer them to the instrument under test using a micropipette or syringe. Each vial contains 1.5 ml of DNACON solution.

The DNACON reference from Starna was developed as a reliable, stable and NIST traceable reference material for the validation of this method.  The reference consists of a solution of a synthetic material whose spectral characteristics closely mimic those of pure DNA, but does not suffer from the instability and other difficulties associated with DNA itself. Two concentrations are available in vial format, to give absorbances of approximately 1 A or 0.5 A at a nominal path length of 1mm.

 

 DNACON Microvolume References

 

DNACON

 Absorbance values are certified at 260 nm, 280 nm and 330 nm for spectral bandwidths of 1.0, 2.0, 3.0, 4.0 and 5.0 nm . The value at 330 nm is used as a “background” correction, as in the normal methodology. From these values a corrected 260/280 ratio is calculated and reported for each bandwidth using the formula:

Ratio =   (A260 – A330)
                    (A280 – A330)

The typical calculated ratio is 1.9

 

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO/IEC 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.