Purpose

 

These reference materials have been specially developed for the validation of DNA purity measurements when performed with the dedicated low volume (< 5 ul) and short path length (< 1 mm) instrumentation offered by several vendors for use in genomics and proteomics, when sample is often at a premium. They are also ideal for qualifying conventional instruments when they are used with ultra- low volume cells such as the Starna DMV - Bio cell.

Description and Discussion

 

Dedicated low-volume instrumentation using drop technology or other measurement techniques is usually incompatible with reference materials presented inconventional spectrophotometer cells or as solid filters. Nevertheless quality control is just as important in this field,and these specially packaged versions of established Starna reference materials are designed to meet this need. The references are supplied in vials with screw-cap closure for ease of handling, from which it is easy to transfer them to the instrument under test using a micropipette or syringe.

The DNACON reference from Starna was developed as a reliable, stable and NIST traceable reference material for the validation of this method. The reference consists of a solution of a synthetic material whose spectral characteristics closely mimic those of pure DNA, but does not suffer fromthe instability and other difficulties associated with DNAitself. Two concentrations are available in vial format, to give absorbances of approximately 1 A or 0.5 A at a nominal path length of 1mm.

 

Typical spectra

 

DNACON

Absorbance values are certified at 260 nm, 280 nm and 330 nm for spectral bandwidths of 1.0, 2.0, 3.0, 4.0 and 5.0 nm . The value at 330 nm is used as a “background” correction, as in the normal methodology. From these values a corrected 260/280 ratio is calculated and reported for each bandwidth using the formula:

Ratio =   (A260 – A330)
                    (A280 – A330)

The typical calculated ratio is 1.9

 

 

Catalogue Number

 

  Catalogue Number 

Starna Microvolume DNACON Reference, 5X concentration: 1 x 1,5ml vial

DNACON5X –SC1 

2 x 1.5 ml vials

DNACON5X –SC2

4 x 1.5 ml vials

DNACON5X -SC4

Starna Microvolume DNACON Reference, 10X concentration :1 x 1,5ml vial

DNACON10X –SC1

2 x 1.5 ml vials

DNACON10X –SC2

4 x 1.5 ml vials

DNACON10X –SC4