These materials may be used on a routine basis to calibrate and monitor the performance of Fluorescence Spectrophotometers.

Six polymer blocks containing seven fluorescent compounds whose spectra cover a broad spectral range with emission maxima from 330 to 582nm and excitation maxima from 290 to 562 nm. This wide spectral range enables the user to select a reference material with broadly similar spectral properties to those of the analyte.




Compound  Approximate
Molar Conc
Wavelength (nm)
Wavelength (nm)
Anthracene  1 x 10-5  360 402
Napthalene  6 x 10-5  290 330
Ovalene  2 x 10-7  343 502
p-Terphenyl  5 x 10-7  295 338
Tetraphenylbutadiene  3 x 10-7  348 422
Compound 610 1 x 10-6  440 475
Rhodamine 2 x 10-7  562 573
Europium III
1 x 10-2  340 615

Provided care is exercised in interpreting the results, these spectra can be successfully used for:

  • Determinations at much higher sensitivity than with absorption methods
  • Quantum yield and energy yield calculations
  • Compound identification
  • Inter-laboratory studies
  • Publication of results
  • Wavelength calibration of spectrofluorometers

A further reference containing Europium III is available for evaluating phosphorescence measurements. Europium III exhibits fluorescence with a lifetime of around 2mS. Reference spectra are provided using excitation and emission wavelengths of 340 nm and 615 nm respectively. Decay rate and fluorescence lifetime are calculated and reported for gate times of 50 µS and 2 µS.



Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.