Purpose

These materials may be used on a routine basis to calibrate, compare and monitor the performance of Fluorescence Spectrophotometers. p-Terphenyl, Ovalene, Tetraphenylbutadiene and Anthracene in PMMA are cited in USP chapter for wavelength validation.

Description and Discussion

Six polymer blocks containing seven fluorescent compounds whose spectra cover a broad spectral range with excitation range from 260 to 600nm and emission range from 370 to 630 nm. This wide spectral range enables the user to select a reference material with broadly similar spectral properties to those of the analyte, ensuring a reasonable spectral overlap and measurable signals without changing important parameters such as slit widths and wavelength settings.

REF NO COMPOUND  APPROXIMATE MOLAR CONCENTRATION  PEAK EXCITATION WAVELENGTH (NM)  EXCITATION RANGE (NM)  PEAK EMISSION WAVELENGTH (NM)  EMISSION RANGE (NM) 
1 Anthracene 1 x 10-5 360  260 - 320  402  370 - 470 
  Napthalene 6 x 10-5 290  290 - 390  330  310 - 400 
2 Ovalene 2 x 10-7 342  300 - 460  482  450 - 570 
3 p-Terphenyl 5 x 10-7 295  270 - 350  338  310 - 410 
4 Tetraphenylbutadiene 3 x 10-7 348  290 - 390  422  370 - 540 
5 Compound 610 1 x 10-6 440  360 - 490  475  420 - 570 
6 Rhodamine 2 x 10-7 562  500 - 600  573  530 - 630 

 The solid polymethyl methacrylate (PMMA) matrix provides a stable environment for the fluorescent compounds. Being solids, there can be no evaporation of solvents, providing long term stability.

Typical spectra

 

Catalogue Number

COMPOUND Catalogue  Number
Set of 6 PMMA Fluorescence References 6BF
Napthalene/Anthracene 6BF-STD/1
Ovalene  6BF-STD/2
p-Terphenyl  6BF-STD/3
Tetraphenylbutadiene 6BF-STD/4
Compound 610  6BF-STD/5
Rhodamine B  6BF-STD/6